extraction and identification of antibacterial secondary metabolites from marine streptomyces sp. vitbrk2

نویسندگان

kannabiran krishnan division of biomolecules and genetics, school of biosciences and technology, vit university, india.

benita mercy division of biomolecules and genetics, school of biosciences and technology, vit university, india.

چکیده

actinomycetes were isolated from marine sediment samples collected from the east coast of chennai, tamil nadu, india. well diffusion and agar plug methods were used for the evaluation of antibiotic production by these isolates against drug resistant methicillin- resistant staphylococcus aureus (mrsa) and vancomycin resistant enterococci (vre). the potential isolate vitbrk2 was mass cultured for morphological and physiological characterization. the culturing conditions of the isolate were optimized and the recommendations of international streptomyces project were followed for the assimilation of carbon and nitrogen sources. the isolate was identified by comparing the properties with representative species in the key of nonomura and bergey’s manual of determinative bacteriology. ethyl acetate extract prepared from the cell free culture broth of the isolate was analyzed using hplc- diode array technique to characterize the metabolites and identify the antibiotics. vitbrk2 was found to be gram-positive rod grey color aerial mycelium production. it was also non motile in nature with spiral spore chain morphology. vitbrk2 was identified as streptomyces and designated as streptomyces sp. vitbrk2. hplc-dad analysis showed the presence of indolo compounds (3- methyl-indole and 2-methyl- indole) along with amicoumacin antibiotic. the observed activity of streptomyces sp. vitbrk2 against mrsa and vre strains may be due to the presence of indolo compounds in the isolate. the results of this study suggested that secondary metabolites produced by streptomyces sp. vitbrk2 could be used as a lead to control drug resistant bacterial pathogens.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Extraction and Identification of Antibacterial Secondary Metabolites From Marine Streptomyces sp. VITBRK2

Actinomycetes were isolated from marine sediment samples collected from the east coast of Chennai, Tamil Nadu, India. Well diffusion and agar plug methods were used for the evaluation of antibiotic production by these isolates against drug resistant Methicillin- resistant Staphylococcus aureus (MRSA) and vancomycin resistant Enterococci (VRE). The potential isolate VITBRK2 was mass cultured for...

متن کامل

Extraction and Identification of Antibacterial Secondary Metabolites from Marine Streptomyces sp. VITBRK2

Actinomycetes were isolated from marine sediment samples collected from the east coast of Chennai, Tamil Nadu, India. Well diffusion and agar plug methods were used for the evaluation of antibiotic production by these isolates against drug resistant Methicillin- resistant Staphylococcus aureus (MRSA) and vancomycin resistant Enterococci (VRE). The potential isolate VITBRK2 was mass cultured for...

متن کامل

Bioactive Secondary Metabolites from a New Terrestrial Streptomyces sp. TN262

During our search for Streptomyces spp. as new producers of bioactive secondary metabolites, the ethyl acetate extract of the new terrestrial Streptomyces isolate TN262 delivered eight antimicrobially active compounds. They were identified as 1-acetyl-beta-carboline (1), tryptophol (2), cineromycin B (3), 2,3-dihydrocineromycin B (4), cyclo-(tyrosylprolyl) (5), 3-(hydroxyacetyl)-indole (6), bre...

متن کامل

Antioxidant and cytotoxic activities of metabolites produced by a new marine Streptomyces sp. isolated from the sea cucumber Holothuria leucospilota

Marine microorganisms are important sources for novel natural products. Hence, the aim of this study was introducing the marine microorganisms with capability to produce antioxidant and cytotoxic metabolites. For this purpose, ten live sea cucumbers (Holothuria leucospilota) were collected from Larak Island. Then, their intestine contents were serially diluted and treated by heating (55 °c). 10...

متن کامل

Antioxidant and cytotoxic activities of metabolites produced by a new marine Streptomyces sp. isolated from the sea cucumber Holothuria leucospilota

Marine microorganisms are important sources for novel natural products. Hence, the aim of this study was to introduce marine microorganisms with the capability of producing antioxidant and cytotoxic metabolites. For this purpose, ten live sea cucumbers (Holothuria leucospilota) were collected from Larak Island, Persian Gulf. Then, their intestine contents were serially diluted and treated by he...

متن کامل

Antibacterial Secondary Metabolites from the Cave Sponge Xestospongia sp

Chemical investigation of the cave sponge Xestospongia sp. resulted in the isolation of three new polyacetylenic long chain compounds along with two known metabolites. The structures of the new metabolites were established by NMR and MS analyses. The antibacterial activity of the new metabolites was also evaluated.

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
international journal of molecular and cellular medicine

جلد ۳، شماره ۳، صفحات ۱۳۰-۱۳۷

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023